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Review
. 2014 Dec;25(12):989-94.
doi: 10.1089/hum.2014.2542.

Retroviral vectors: from cancer viruses to therapeutic tools

A Dusty Miller  1
Affiliations
Review

Retroviral vectors: from cancer viruses to therapeutic tools

A Dusty Miller. Hum Gene Ther. 2014 Dec.
No abstract available

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Figures

<b>FIG. 1.</b>
FIG. 1.
Design of retroviral packaging cell lines to avoid recombination leading to helper virus production. (A) Schematic diagram of the MoMLV replication-competent (helper) retrovirus is shown at top with a scale bar (1 kb) at bottom left. (B–E) Evolving designs for retroviral packaging cell lines. Regions where recombination might occur between packaging cell line components and retroviral vectors to generate helper virus are shown by purple boxes, with Z-shaped lines indicating the direction of recombination. Directional arrows indicate sites of transcription initiation and bidirectional arrows at top indicate the extent of the retroviral protein coding regions. ψ, retroviral RNA packaging signal; ψ+, extended retroviral RNA packaging signal; ATG, gag coding region start codon; LTR, retroviral long terminal repeat; pA, polyadenylation signal; SA, splice acceptor; SD, splice donor; TAG, mutation of the gag start codon to a stop codon to prevent Gag protein translation.
See this image and copyright information in PMC

References

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    1. Battini J.L., Rasko J.E., and Miller A.D. (1999). A human cell-surface receptor for xenotropic and polytropic murine leukemia viruses: possible role in G protein-coupled signal transduction. Proc. Natl. Acad. Sci. USA 96, 1385–1390 - PMC - PubMed

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