Establishment of a model of murine odontoblasts underexpressing PKD1 using shRNA

Abstract

We have previously shown that PKD1, the gene encoding Polycystin-1 (or TRPP1) is expressed in human odontoblasts and that this protein is localized at the primary cilium of the cell. Nevertheless, its function remain unclear in this cell even if studies on osteoblasts, osteocytes and chondrocytes give TRPP1 as a promising candidate for mechanotransduction in response to mechanical stress. Consequently, to evaluate the role of TRPP1 in this transduction process, we needed first to generate an in vitro murine model down expressing Pkd1. Using lentivirus-mediated shRNA technology, we obtained a 60% suppression of Pkd1 mRNA expression in transfected MO6-G3 cells associated with a decrease of cell proliferation. Thus, establishment of this murine odontoblast model underexpressing Pkd1 associated with applied mechanical forces (compression or shear stress) will allow us to go further in the determination of TRPP1 involvement in odontoblasts mechanotransduction.