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. 2015 Jan:108:36-44.
doi: 10.1016/j.mimet.2014.11.007. Epub 2014 Nov 20.

Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model

Julie A K McDonald  1 , Susana Fuentes  2 , Kathleen Schroeter  1 , Ineke Heikamp-deJong  2 , Cezar M Khursigara  1 , Willem M de Vos  2 , Emma Allen-Vercoe  3
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Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model

Julie A K McDonald et al. J Microbiol Methods. 2015 Jan.

Abstract

In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.

Keywords: Biofilm; Chemostat; DGGE; HITChip; Human gut microbiota; Microbial ecology.

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