Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Dec;165(10):803-12.
doi: 10.1016/j.resmic.2014.10.006. Epub 2014 Oct 22.

Characterization of virus-like particles associated with the human faecal and caecal microbiota

Lesley Hoyles  1 , Anne L McCartney  2 , Horst Neve  3 , Glenn R Gibson  4 , Jeremy D Sanderson  5 , Knut J Heller  6 , Douwe van Sinderen  7
Affiliations
Free article

Characterization of virus-like particles associated with the human faecal and caecal microbiota

Lesley Hoyles et al. Res Microbiol. 2014 Dec.
Free article

Abstract

This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.

Keywords: Bacteriophages; Caecum; Faeces; Gastrointestinal tract; Microbial ecology; Transmission electron microscopy.

PubMed Disclaimer

Publication types