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. 2015 May;119(5):538-42.
doi: 10.1016/j.jbiosc.2014.10.013. Epub 2014 Nov 15.

Isolation of the stable strain Labrys sp. BK-8 for L(+)-tartaric acid production

Wenna Bao  1 , Haifeng Pan  1 , Zhenhong Zhang  2 , Yongqing Cheng  2 , Zhipeng Xie  3 , Jianguo Zhang  1
Affiliations

Isolation of the stable strain Labrys sp. BK-8 for L(+)-tartaric acid production

Wenna Bao et al. J Biosci Bioeng. 2015 May.

Abstract

A novel cis-epoxysuccinate hydrolase (CESH) producing strain of Labrys sp. BK-8 for production of L(+)-tartaric acid was isolated and identified. After optimization, a maximum activity of 3597 ± 151 U/g was achieved in batch culture in a 10 L fermentor. When Labrys sp. BK-8 was immobilized on κ-carrageenan, the immobilized cells showed a high conversion rate (>99%), enantioselectivity (EE > 99.5%) and storage stability (>90 d). A conversion rate of 97% was still achieved after 10 repeat batches. The CESH was stable over a broad range of temperatures (up to 45°C) and pH values (4.0-10.0). The Labrys sp. BK-8 isolate provides a new alternative with good stability for the industrial biosynthesis of L(+)-tartaric acid.

Keywords: Immobilization; Isolation; Labrys sp.; cis-Epoxysuccinate hydrolase; l(+)-Tartaric acid.

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