Comparative Study
doi: 10.1002/jcla.1860030307.
IgM to human cytomegalovirus: comparison of two enzyme immunoassays and IgM reactivity to viral polypeptides detected by immunoblotting
Affiliations
- PMID: 2547041
- DOI: 10.1002/jcla.1860030307
Item in Clipboard
Comparative Study
IgM to human cytomegalovirus: comparison of two enzyme immunoassays and IgM reactivity to viral polypeptides detected by immunoblotting
J Clin Lab Anal.
1989.
Abstract
IgM detection by enzyme-linked immunosorbent assay (ELISA) is the most used method of establishing a cytomegalovirus (CMV) infection during pregnancy. In this paper, we discuss the results obtained assaying 1,000 sera from pregnant women by two ELISA kits: a traditional indirect ELISA and a more recent IgM-capture ELISA. All the sera that gave a positive ELISA value with one or both kits were further tested by immunoblotting (IB) to establish which CMV polypeptides were detected by IgM antibodies. From the results obtained, IgM-capture ELISA seems less sensitive than indirect ELISA, but correlates better with serological evidence of active CMV infections as judged by typical IB profiles.
Similar articles
-
Evaluation of two novel enzyme immunoassays using recombinant antigens to detect cytomegalovirus-specific immunoglobulin M in sera from pregnant women.J Clin Virol. 1999 Aug;13(3):161-71. doi: 10.1016/s1386-6532(99)00028-1. J Clin Virol. 1999. PMID: 10443792
-
Immunoglobulin M antibodies detected by enzyme-linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infections in pregnant women and newborn infants.J Clin Microbiol. 1985 Jun;21(6):930-5. doi: 10.1128/jcm.21.6.930-935.1985. J Clin Microbiol. 1985. PMID: 2989326 Free PMC article.
-
Evaluation of the indirect and IgM-capture anti-human cytomegalovirus IgM ELISA methods as confirmed by cytomegalovirus IgG avidity.Microbiol Immunol. 2019 May;63(5):172-178. doi: 10.1111/1348-0421.12683. Epub 2019 May 22. Microbiol Immunol. 2019. PMID: 31012489
-
[Cytomegalovirus infection: pathogenesis, diagnosis and prevention].Dtsch Med Wochenschr. 1986 Aug 15;111(33):1251-7. doi: 10.1055/s-2008-1068616. Dtsch Med Wochenschr. 1986. PMID: 3017671 Review. German. No abstract available.
-
A user's guide to the indirect solid-phase radioimmunoassay for the detection of cytomegalovirus-specific IgM antibodies.J Virol Methods. 1984 Jul;8(4):271-82. doi: 10.1016/0166-0934(84)90064-8. J Virol Methods. 1984. PMID: 6088564 Review.
Cited by
-
Lack of correlation between virus detection and serologic tests for diagnosis of active cytomegalovirus infection in patients with AIDS.J Clin Microbiol. 1992 Apr;30(4):1027-9. doi: 10.1128/jcm.30.4.1027-1029.1992. J Clin Microbiol. 1992. PMID: 1315328 Free PMC article.
-
Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay.Clin Diagn Lab Immunol. 1996 Sep;3(5):597-600. doi: 10.1128/cdli.3.5.597-600.1996. Clin Diagn Lab Immunol. 1996. PMID: 8877143 Free PMC article.
-
Antibody responses to synthetic peptides from cytomegalovirus phosphoprotein 150.J Clin Microbiol. 1992 Oct;30(10):2735-9. doi: 10.1128/jcm.30.10.2735-2739.1992. J Clin Microbiol. 1992. PMID: 1328283 Free PMC article.
-
Cross-reactivity of Epstein-Barr virus-specific immunoglobulin M antibodies with cytomegalovirus antigens containing glycine homopolymers.Clin Diagn Lab Immunol. 2001 Jul;8(4):747-56. doi: 10.1128/CDLI.8.4.747-756.2001. Clin Diagn Lab Immunol. 2001. PMID: 11427421 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
