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. 2014 Nov;7(2):135-42.
doi: 10.15283/ijsc.2014.7.2.135.

Characterization of mesenchymal stem cells derived from rat bone marrow and adipose tissue: a comparative study

Ahmed Lotfy  1 , Mohamed Salama  2 , Faten Zahran  3 , Elena Jones  4 , Ahmed Badawy  2 , Mohamed Sobh  2
Affiliations

Characterization of mesenchymal stem cells derived from rat bone marrow and adipose tissue: a comparative study

Ahmed Lotfy et al. Int J Stem Cells. 2014 Nov.

Abstract

Background and objectives: Stem cell technology offers a new hope for many chronic disorders patients. The types of stem cells are different with many differences existing between each type. Mesenchymal stem cells (MSCs) represent one type of adult stem cells that can be easily isolated, then re-transplanted to the patients. This offers potential for their future application in treating many disorders without fear of rejection possibility. MSCs can be isolated from different sources e.g. bone marrow (BMSCs) and adipose tissue (ADSCs). In the present study we compared BMSCs and ADSCs isolated from Sprague-Dawley rats.

Methods and results: For this comparison, immunophenotyping, the analysis of growth rates, proliferation by colony forming unit-fibroblast assay, population doubling time, and trilineage differentiation assays were performed for both BMSCs and ADSCs. The findings revealed that despite no difference in immunphenotypic character between BMSC and ADSC, a better proliferative capacity was observed for ADSCs which would advocate their better use in regenerative applications. On the other hand, BMSCs showed more potential for osteogenic and chondrogenic differentiation.

Conclusions: Our study showed that, despite many similarities between both types of cells, there are differences existing which can offer assistance on choosing type of cell to be used in specific diseases. Although ADSCs seem more promising for regenerative application generally, BMSCs may represent a better choice for treating bone disorders.

Keywords: ADSCs; BMSCs; Bone Marrow; Characterization; Mesenchymal Stem Cells; Sprague-Dawley Rats.

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Figures

Fig. 1.
Fig. 1.
Image of BMSCs in passage 3 with 80% confluence (A) 10X (B) 20X. Image of ADSCs in passage 3 with 80% confluence (C) 10X. (D) 20X. (E) ADSCs show ability to form multilayer after confluent 20X.
Fig. 2.
Fig. 2.
(A) Number of CFU.F for BMSCs & ADSCs when plated per 100-mm tissue culture dish. (B) PDT per hours for BMSCs & ADSCs. (C) Growth curve plotted for BMSCs & ADSCs.
Fig. 3.
Fig. 3.
(A, B) Osteogenic Differentiation of (A) BMSCs and (B) ADSCs. Both stained with alizarin red. (C, D) Adipogenic Differentiation of (C) BMSCs and (D) ADSCs stained with oil red. (E) Chondrogenic Differentiation, this chart showing level of sulfated GAG for both BMSCs and ADSCs.
See this image and copyright information in PMC

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