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. 2014 Oct 9;45(3):841-3.
doi: 10.1590/s1517-83822014000300012. eCollection 2014.

Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

F L E Spositto  1 , P A Z Campanerut  2 , L D Ghiraldi  2 , C Q F Leite  3 , M H Hirata  4 , R D C Hirata  4 , V L D Siqueira  2 , R Fressatti Cardoso  2
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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

F L E Spositto et al. Braz J Microbiol. .

Abstract

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

Keywords: PCR-multiplex; bovine tuberculosis; diagnosis; tuberculosis.

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Figures

Figure 1
Figure 1
Specificity of multiplex-PCR for differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC). Electrophoresis on 2% agarose gel. Line M: DNA molecular weight marker (100 bp); Line 1: M. tuberculosis H37Rv; Line 2: M. bovis AN5; Line 3: M. bovis BCG; Line 4: M. avium; Line 5: M. kansasii; Line 6: M. abcessus; Line 7: M. chelonae; Line 8: M. massiliense; Line 9: M. szulgai; Line 10: M. fortuitum; Line 11: negative control.
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