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. 2014 Oct 9;45(3):1023-30.
doi: 10.1590/s1517-83822014000300035. eCollection 2014.

Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria

Judit Süle  1 , Tímea Kõrösi  2 , Attila Hucker  2 , László Varga  1
Affiliations

Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria

Judit Süle et al. Braz J Microbiol. .

Abstract

The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or exceeding those of Lb. acidophilus. Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selective enumeration of Lactobacillus spp.

Keywords: Bifidobacterium; Lactobacillus acidophilus; ciprofloxacin; clindamycin; mupirocin.

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