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. 2014 Oct;8(10):ZC56-60.
doi: 10.7860/JCDR/2014/8239.5042. Epub 2014 Oct 20.

Estimation of levels of salivary mucin, amylase and total protein in gingivitis and chronic periodontitis patients

Swati Kejriwal  1 , Rahul Bhandary  2 , Biju Thomas  3 , Suchetha Kumari  4
Affiliations

Estimation of levels of salivary mucin, amylase and total protein in gingivitis and chronic periodontitis patients

Swati Kejriwal et al. J Clin Diagn Res. 2014 Oct.

Abstract

Background: Periodontal diseases are a group of inflammatory conditions resulting from interaction between a pathogenic bacterial biofilm and susceptible host's inflammatory response eventually leading to the destruction of periodontal structures and subsequent tooth loss. Hence, investigation of salivary proteins in individuals with periodontal diseases may be useful to enhance the knowledge of their roles in these diseases.

Materials and methods: This case-control study was conducted at A.B. Shetty Memorial Institute of Dental Sciences, Mangalore. The study comprised of 90 patients of age between 25-60 years who were clinically examined and divided into three groups of 30 each: namely clinically healthy, gingivitis and chronic periodontitis. These were classified according to the values of gingival index score, clinical attachment loss and probing pocket depth. Unstimulated saliva was collected and salivary mucin, amylase and total protein levels were determined.

Statistical analysis: RESULTS obtained were tabulated and statistically analyzed using ANOVA test and Karl pearson's correlation test.

Results: The results of the study showed an increased concentration of salivary mucin, amylase and total protein in gingivitis patients and increased levels of amylase and total protein in saliva of chronic periodontitis patients compared to healthy individuals which were statistically significant. A decrease in mucin concentration was observed in the periodontitis group compared to gingivitis group. A positive correlation was present between salivary mucin, amylase and total protein levels in the three groups.

Conclusion: Salivary mucin, amylase and total protein may serve as an important biochemical parameter of inflammation of the periodontium. Also, it can be hypothesized that various enzyme inhibitors might be useful as a part of host modulation therapy in the treatment of periodontal diseases.

Keywords: Enzyme; Glycoprotein; Host modulation; Saliva.

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Figures

[Table/Fig-2]:
[Table/Fig-2]:
Comparison of mean salivary amylase levels (U/ml) between healthy, gingivitis and chronic periodontitis patients
[Table/Fig-3]:
[Table/Fig-3]:
Comparison of mean salivary total protein levels (mg/ml) between healthy, gingivitis and chronic periodontitis patients
[Table/Fig-4]:
[Table/Fig-4]:
Comparison of mean salivary mucin levels (mg/ml) between healthy, gingivitis and chronic periodontitis patients.
[Table/Fig-6]:
[Table/Fig-6]:
Shows significant positive correlation between salivary mucin and amylase levels
[Table/Fig-7]:
[Table/Fig-7]:
Shows significant positive correlation between salivary mucin and total protein levels
[Table/Fig-8]:
[Table/Fig-8]:
Shows significant positive correlation between salivary amylase and total protein levels
See this image and copyright information in PMC

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References

    1. Rocha Dde M, Zenóbio EG, Van Dyke T, Silva KS, Costa FO, Soares RV. Differential expression of salivary glycoproteins in aggressive and chronic periodontitis. J Appl Oral Sci. 2012;20(2):180–85. - PMC - PubMed
    1. Gonçalves Lda R, Soares MR, Nogueira FC, Garcia C, Camisasca DR, Domont G, et al. Comparative proteomic analysis of whole saliva from chronic periodontitis patients. J Proteomics. 2010;73:1334–41. - PubMed
    1. Gonçalves Lda R, Soares MR, Nogueira FC, Garcia CH, Camisasca DR, Domont G, et al. Analysis of the salivary proteome in gingivitis patients. J Periodontal Res. 2011;46(5):599–606. - PubMed
    1. Giannobile WV, Beikler T, Kinney JS, Ramseier CA, Morelli T, Wong DT. Saliva as a diagnostic tool for periodontal disease: current state and future directions. Periodontol. 2000. 2009;50:52–64. - PMC - PubMed
    1. Sánchez GA, Miozza V, Delgado A, Busch L. Determination of salivary levels of mucin and amylase in chronic periodontitis patients. J Periodontal Res. 2011;46(2):221–27. - PubMed