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. 1989 Aug 15;122(1):97-103.
doi: 10.1016/0022-1759(89)90339-6.

Purification of human blood eosinophils by negative selection using immunomagnetic beads

T T Hansel  1 , J D Pound, D Pilling, G D Kitas, M Salmon, T A Gentle, S S Lee, R A Thompson
Affiliations

Purification of human blood eosinophils by negative selection using immunomagnetic beads

T T Hansel et al. J Immunol Methods. .

Abstract

A simple method for isolating highly purified eosinophils from human blood is described. Buffy coats from normal individuals (eosinophil counts less than 0.4 x 10(9)/litre) were centrifuged through a two layer Percoll density gradient, to produce a granulocyte fraction containing neutrophils and eosinophils. Neutrophils were extracted from this fraction using a monoclonal antibody (CLB FcR gran 1) against CD 16 (Fc gamma R III) in a direct or indirect selection procedure using immunomagnetic beads (Dynabeads). This negative immunoselection produced eosinophils of greater purity and with a superior capacity to mount a respiratory burst than eosinophils isolated by a method employing metrizamide.

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