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. 2015 Apr:32:37-42.
doi: 10.1016/j.anaerobe.2014.12.003. Epub 2014 Dec 4.

Mageeibacillus indolicus gen. nov., sp. nov.: a novel bacterium isolated from the female genital tract

Michele N Austin  1 , Lorna K Rabe  2 , Sujatha Srinivasan  3 , David N Fredricks  3 , Harold C Wiesenfeld  4 , Sharon L Hillier  4
Affiliations

Mageeibacillus indolicus gen. nov., sp. nov.: a novel bacterium isolated from the female genital tract

Michele N Austin et al. Anaerobe. 2015 Apr.

Abstract

Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C14:0, C16:0, C18:2 ω6, 9c, C18:1 ω9c and C18:0 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, Saccharofermentans acetigenes. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order Clostridiales with an association with Clostridium rRNA cluster III within the family Ruminococcaceae. We propose the name, Mageeibacillus indolicus gen. nov., sp. nov. The type strain is BAA-2120(T) and CCUG 59143(T).

Keywords: Bacterial vaginosis associated bacterium 3; Genital tract; Mageeibacillus indolicus gen. nov., sp. nov.; Taxonomy.

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Figures

Figure 1
Figure 1
Neighbor-joining phylogenetic dendrogram showing the phylogenetic position of Mageeibacillus indolicus CCUG 59143T among closely related species using 16S rRNA sequences. The tree was rooted with C. butyricum ATCC 19398T and C. perfringes ATCC 13124T. Numbers at branch points represent bootstrap support (%) based on an analysis of 1000 resampled datasets. GenBank accession numbers of the 16S rRNA gene sequences are provided in parentheses. Bar, 2% sequence divergence.
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