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. 1989 Jun 1;260(2):593-6.
doi: 10.1042/bj2600593.

The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation

S Palmer  1 , M J Wakelam
Affiliations

The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation

S Palmer et al. Biochem J. .

Abstract

Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.

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References

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