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. 1989 May;8(4):297-9.
doi: 10.1089/dna.1.1989.8.297.

A rapid procedure to identify newborn transgenic mice

C S Lin  1 , T Magnuson, D Samols
Affiliations

A rapid procedure to identify newborn transgenic mice

C S Lin et al. DNA. 1989 May.

Abstract

We have developed a rapid procedure to identify newborn transgenic mice containing foreign genetic material in their genome. The protocol involves collagenase digestion of a small amount of tail tissue which can be taken very early after birth, phenol and chloroform extraction, polymerase chain reaction, and polyacrylamide gel electrophoresis. The entire procedure, from tissue biopsy to final results, can be completed in 1 day.

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