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Comparative Study
. 1989 Aug 11;17(15):6153-65.
doi: 10.1093/nar/17.15.6153.

Ubiquitin expression in Neurospora crassa: cloning and sequencing of a polyubiquitin gene

G E Taccioli  1 , E Grotewold, G O Aisemberg, N D Judewicz
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Free PMC article
Comparative Study

Ubiquitin expression in Neurospora crassa: cloning and sequencing of a polyubiquitin gene

G E Taccioli et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have cloned and sequenced a polyubiquitin gene from Neurospora crassa that is organized in a four repeat-tandem array. The first repeat contains a small intron and the last is fused to an extra glutamine codon. In Northern blots, two RNA species of 1.3 kb and 0.7 kb hybridize to the isolated clone. The larger ubiquitin (UBI) transcript accumulates after partial inhibition of protein synthesis with cycloheximide, and the smaller one preferentially accumulates in conidia after germination. Unexpectedly, constitutive expression of UBI transcripts in exponentially grown mycelia is not altered by heat-shock or exposure to arsenite.

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