doi: 10.1016/0378-1097(89)90495-3.
Transcription initiation and a RNA polymerase binding site upstream of the purE gene of the archaebacterium Methanobacterium thermoautotrophicum strain delta H
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- PMID: 2550316
- DOI: 10.1016/0378-1097(89)90495-3
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Transcription initiation and a RNA polymerase binding site upstream of the purE gene of the archaebacterium Methanobacterium thermoautotrophicum strain delta H
FEMS Microbiol Lett.
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Abstract
DNA-dependent RNA-polymerase (RNAP) purified from the thermophilic archaebacterium Methanobacterium thermoautotrophicum strain delta H has been shown to bind specifically to DNA in the intergenic region upstream of the purE gene cloned from this species. The RNAP binding site has been limited to a 41 bp region of DNA which contains the Box A archaebacterial promoter sequence, 5'ATTAAATA. Transcription of the purE gene appears to initiate in vivo at three locations 20-22 bp downstream of the Box A sequence and 27-29 bp upstream of the ATG translation initiation codon of the purE gene.
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