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Comparative Study
. 1989 Sep;27(9):2115-7.
doi: 10.1128/jcm.27.9.2115-2117.1989.

Comparison of three kinds of blood and two incubation atmospheres for cultivation of Bordetella pertussis on charcoal agar

J E Hoppe  1 , M Schlagenhauf
Affiliations
Comparative Study

Comparison of three kinds of blood and two incubation atmospheres for cultivation of Bordetella pertussis on charcoal agar

J E Hoppe et al. J Clin Microbiol. 1989 Sep.

Abstract

We compared the growth of Bordetella pertussis strains (n = 32) on antibiotic-free and cephalexin (40 micrograms/ml)-containing charcoal agar supplemented with 10% defibrinated horse blood, defibrinated sheep blood, or anticoagulant-containing human blood. Plates were incubated either in air or in an atmosphere with 5 to 10% CO2. As assessed by mean colony numbers and rapidity of growth, normal air was preferable to CO2 enrichment for incubation. Growth on horse blood agar was more abundant and more rapid than on sheep blood agar, but the difference in general was not statistically significant. Human blood was clearly inferior to both horse and sheep blood.

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References

    1. J Clin Microbiol. 1984 Nov;20(5):891-3 - PubMed
    1. Eur J Clin Microbiol Infect Dis. 1988 Oct;7(5):616-20 - PubMed
    1. J Clin Microbiol. 1987 Jun;25(6):1109-10 - PubMed

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