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. 2014 Oct;37(4):646-51.
doi: 10.1590/S1415-47572014005000024. Epub 2014 Nov 14.

Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers

Fabilene Gomes Paim  1 , José Henrique Souza Galdino Brandão  1 , Iracilda Sampaio  2 , Paulo Roberto Antunes de Mello Affonso  1 , Débora Diniz  1
Affiliations

Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers

Fabilene Gomes Paim et al. Genet Mol Biol. 2014 Oct.

Abstract

Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of heterochromatin and GC-rich single nucleolar organizer regions (NORs) not syntenic to 5S rDNA clusters. These are the first data about DNA barcoding in parrotfish from the Brazilian province and the first refined chromosomal analysis in Scarinae, providing useful data to a reliable genetic identification of S. radians.

Keywords: COI; Robertsonian rearrangements; South Atlantic fish; karyotype; ribossomal genes.

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Figures

Figure 1
Figure 1
Neighbor-Joining tree of COI sequences comparing S. radians from Brazilian coast and other populations and species of Sparisoma. The numbers in parentheses refer to GenBank database (see text for details).
Figure 2
Figure 2
Karyotype of Sparisoma radians (2n = 46) after Giemsa staining (A) and C-banding (B). The NOR-bearing pair is shown in detail after silver nitrate (C) and fluorochrome staining (D) revealing CMA3+ sites coincident with NORs.
Figure 3
Figure 3
Distribution of 18S rDNA (in green) and 5S rDNA (in magenta) in DAPI-stained karyotype of S. radians after double-FISH.
See this image and copyright information in PMC

References

    1. Accioly IV, Bertollo L, Costa G, Jacobina U, Molina WF. Chromosomal population structuring in carangids (Perciformes) between the north-eastern and south-eastern coasts of Brazil. Afr J Mar Sci. 2012;34:383–389.
    1. Bellwood DR. A phylogenetic study of the parrotfishes family Scaridae (Pisces, Labroidei), with a revision of genera. Rec Aust Mus. 1994;20:1–86.
    1. Bernardi G, Robertson DR, Clifton KE, Azzurro E. Molecular systematics, zoogeography, and evolutionary ecology of the Atlantic parrotfish genus Sparisoma. Mol Phylogenet Evol. 2000;15:292–300. - PubMed
    1. Feitoza BM, Rosa RS, Rocha LA. Ecology and zoogeography of deep–reef fishes in Northeastern Brazil. Bull Mar Sci. 2005;76:725–742.
    1. Galetti PM, Jr, Molina WF, Affonso PRAM, Aguilar CT. Assessing genetic diversity of Brazilian reef fishes by chromosomal and DNA markers. Genetica. 2006;126:161–177. - PubMed