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. 2014 Nov;17(6):571-4.
doi: 10.4103/0972-0707.144607.

Disinfection of gutta-percha cones using three reagents and their residual effects

Mahesh Martur Chandrappa  1 , Nofal Mundathodu  1 , Raghu Srinivasan  1 , Farhat Nasreen  1 , Pamarthi Kavitha  1 , Ashwija Shetty  1
Affiliations

Disinfection of gutta-percha cones using three reagents and their residual effects

Mahesh Martur Chandrappa et al. J Conserv Dent. 2014 Nov.

Abstract

Aims and objectives: The purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and MTAD in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure.

Materials and methods: Gutta-percha cones were immersed in suspensions of Enterococcus faecalis and Staphylococcus aureus separately. The cones were then immersed in 5.25% NaOCl, 2% CHX and MTAD for 30 seconds, 1 minute and 5 minutes separately. The disinfected cones were then incubated in thioglycollate media for 7 days. The thioglycolate media was sub-cultured and colony forming units were counted. For topographical examination of gutta-percha cones, the cones were immersed in respective solutions for one minute and allowed to air dry for 30 minutes after rinsing or without rinsing the cones with distilled water. The topography of the cones were then analyzed under SEM. Data was statistically analyzed using one way anova.

Results: MTAD was found to be the most effective disinfecting solution. The topographical examination of GP cones found some deposits after the disinfection procedure in every group. These deposits were removed when the GP cones were rinsed with distilled water.

Conclusions: MTAD possesses superior bactericidal activity when compared with NaOCl and CHX and a final rinse with distilled water is essential after the disinfection procedure.

Keywords: Disinfection; efficacy; topography.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
SEM image of gutta-percha cones before and after immersing in disinfecting solutions for 1 minute, (a) Control group (b) 5.25% NaOCl — Before rinse (c) 5.25% NaOCl — After rinse (d) 2% CHX — Before rinse (e) 2% CHX — After rinse (f) MTAD — Before rinse (g) MTAD — After rinse
See this image and copyright information in PMC

References

    1. Kayaoglu G, Gürel M, Omürlü H, Bek ZG, Sadik B. Examination of gutta percha cones for microbial contamination during chemical use. J Appl Oral Sci. 2009;17:244–7. - PMC - PubMed
    1. Klager P, Dupont AA. The significance of environmental contamination of sealer and gutta-percha before endodontic obturation. Oral Surg Oral Med Oral Pathol. 1987;63:606–9. - PubMed
    1. Gomes BP, Vianna ME, Matsumoto CU, Rossi Vde P, Zaia AA, Ferraz CC, et al. Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;100:512–7. - PubMed
    1. Gajan EB, Aghazadeh M, Abashov R, Salem Milani A, Moosavi Z. Microbial flora of root canals of pulpally-infected teeth: Enterococcus faecalis a prevalent species. J Dent Res Dent Clin Dent Prospects. 2009;3:24–7. - PMC - PubMed
    1. Short RD, Dorn SO, Kuttler S. The crystallization of sodium hypochlorite on gutta-percha cones after the rapid-sterilization technique: An SEM study. J Endod. 2003;29:670–3. - PubMed

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