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. 2014 Dec 17:4:7515.
doi: 10.1038/srep07515.

Synthesis of Au/graphene oxide composites for selective and sensitive electrochemical detection of ascorbic acid

Jian Song  1 , Lin Xu  1 , Ruiqing Xing  1 , Qingling Li  1 , Chunyang Zhou  1 , Dali Liu  1 , Hongwei Song  1
Affiliations

Synthesis of Au/graphene oxide composites for selective and sensitive electrochemical detection of ascorbic acid

Jian Song et al. Sci Rep. .

Abstract

In this work, we present a novel ascorbic acid (AA) sensor applied to the detection of AA in human sera and pharmaceuticals. A series of Au nanoparticles (NPs) and graphene oxide sheets (Au NP/GO) composites were successfully synthesized by reduction of gold (III) using sodium citrate. Then the Au NP/GO composites were used to construct nonenzymatic electrodes in practical AA measurement. The electrode that has the best performance presents attractive analytical features, such as a low working potential of +0.15 V, a high sensitivity of 101.86 μA mM(-1) cm(-2) to AA, a low detection limit of 100 nM, good reproducibility and excellent selectivity. And more,it was also employed to accurately and practically detect AA in human serum and clinical vitamin C tablet with the existence of some food additive. The enhanced AA electrochemical properties of the Au NP/GO modified electrode in our work can be attributed to the improvement of electroactive surface area of Au NPs and the synergistic effect from the combination of Au NPs and GO sheets. This work shows that the Au NP/GO/GCEs hold the prospect for sensitive and selective determination of AA in practical clinical application.

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Figures

Figure 1
Figure 1
TEM images of (a) GO sheets. (b–e) Au NP/GO composites based on S1, S2, S3 and S4. (f) HRTEM image of Au/GO composite based on S3, the inset is the corresponding SEAD pattern.
Figure 2
Figure 2
(a) XRD patterns of Au/GO composites S1, S2, S3 and S4. (b) Raman spectra of GO and Au/GO composites. (c) TGA curves of S1, S2, S3 and S4 samples.
Figure 3
Figure 3
CV curves of (a) Au/GO composite (S3) in 0.1 M PBS solution with the absence (black trace) and presence (green trace) of 1 mM AA. (b) Au/GO composites (S1, S2, S3 and S4) in 0.1 M PBS solution with the present of 1 mM AA. (c) Schematic illustration of AA sensing mechanism by using Au/GO composite.
Figure 4
Figure 4
Amperometric response of (a) S1, S2, S3 and S4 to successive additions of 0.1 mM AA at an applied potential of 0.15 V. (b) The corresponding calibration curves of (a). (c) S3 to the additions of 100 nM AA at an applied potential of +0.15 V. (d) S3 to successive additions of human serum (black trace) and Vitamin C tablet (red trace) contained 100 μM AA. Inset is the corresponding calibration curves of (d).
Figure 5
Figure 5. Amperometric response of S3 at +0.15 V with successive additions of different analytes.
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