Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2015 Mar;53(3):973-5.
doi: 10.1128/JCM.03297-14. Epub 2014 Dec 17.

Evaluation of the Cepheid Xpert C. difficile/Epi and meridian bioscience illumigene C. difficile assays for detecting Clostridium difficile ribotype 033 strains

Grace O Androga  1 , Alan M McGovern  1 , Briony Elliott  1 , Barbara J Chang  1 , Timothy T Perkins  1 , Niki F Foster  1 , Thomas V Riley  2
Affiliations
Comparative Study

Evaluation of the Cepheid Xpert C. difficile/Epi and meridian bioscience illumigene C. difficile assays for detecting Clostridium difficile ribotype 033 strains

Grace O Androga et al. J Clin Microbiol. 2015 Mar.

Abstract

Clostridium difficile PCR ribotype 033 (RT033) is found in the gastrointestinal tracts of production animals and, occasionally, humans. The illumigene C. difficile assay (Meridian Bioscience, Inc.) failed to detect any of 52 C. difficile RT033 isolates, while all strains signaled positive for the binary toxin genes but were reported as negative for C. difficile by the Xpert C. difficile/Epi assay (Cepheid).

PubMed Disclaimer

Figures

FIG 1
FIG 1
Comparison of a complete PaLoc with the virulence genes (tcdA, tcdB) and accessory genes (tcdD, tcdE, tcdC) (A) and the truncated RT033 PaLoc containing parts of tcdA (fragments A2 and A3) and tcdC (B). tcdD, positive regulator gene; tcdE, holin-like-protein-encoding gene; tcdC, negative regulator; tcdA, toxin A gene; tcdB, toxin B gene. (Based on data from reference .)
See this image and copyright information in PMC

References

    1. Magill SS, Edwards JR, Bamberg W, Beldavs ZG, Dumyati G, Kainer MA, Lynfield R, Maloney M, McAllister-Hollod L, Naddle J, Ray SM, Thompson DL, Wilson LE, Fridkin SK. 2014. Multistate point-prevalence survey of health care-associated infections. N Engl J Med 370:1198–1208. doi:10.1056/NEJMoa1306801. - DOI - PMC - PubMed
    1. Stare BG, Delmée M, Rupnik M. 2007. Variant forms of the binary toxin CDT locus and tcdC gene in Clostridium difficile strains. J Med Microbiol 56:329–335. doi:10.1099/jmm.0.46931-0. - DOI - PubMed
    1. Geric B, Johnson S, Gerding DN, Grabnar M, Rupnik M. 2003. Frequency of binary toxin genes among Clostridium difficile strains that do not produce large clostridial toxins. J Clin Microbiol 41:5227–5232. doi:10.1128/JCM.41.11.5227-5232.2003. - DOI - PMC - PubMed
    1. Rupnik M. 2008. Heterogeneity of large clostridial toxins: importance of Clostridium difficile toxinotypes. FEMS Microbiol Rev 32:541–555. doi:10.1111/j.1574-6976.2008.00110.x. - DOI - PubMed
    1. Rupnik M, Brazier JS, Duerden BI, Grabnar M, Stubbs SL. 2001. Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes. Microbiology 147:439–447. - PubMed

MeSH terms

LinkOut - more resources