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Comparative Study
. 2015 Mar;53(3):976-80.
doi: 10.1128/JCM.03444-14. Epub 2014 Dec 17.

Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers

Angèle Gayet-Ageron  1 , Frédéric Laurent  2 , Jacques Schrenzel  3 , Béatrice Charton  2 , Gisela Jimenez-Getaz  4 , Manuela Tangomo  5 , Tristan Ferry  6 , Patrice Sednaoui  7 , Stephan Lautenschlager  8 , Laurence Toutous-Trellu  9 , Begoña Martinez de Tejada  10 , Matthias Cavassini  11 , Stéphane Emonet  4 , Thomas Perneger  12 , Hélène Salord  2
Affiliations
Comparative Study

Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers

Angèle Gayet-Ageron et al. J Clin Microbiol. 2015 Mar.

Abstract

Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.

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