Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Aug;9(8):3342-9.
doi: 10.1128/mcb.9.8.3342-3349.1989.

A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae

G Cottarel  1 , J H Shero, P Hieter, J H Hegemann
Affiliations

A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae

G Cottarel et al. Mol Cell Biol. 1989 Aug.

Abstract

Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, and CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.

PubMed Disclaimer

References

    1. Cell. 1984 May;37(1):67-75 - PubMed
    1. Nature. 1980 Oct 9;287(5782):504-9 - PubMed
    1. Genetics. 1982 May;101(1):17-33 - PubMed
    1. Mol Cell Biol. 1988 Jun;8(6):2523-35 - PubMed
    1. Annu Rev Genet. 1985;19:29-55 - PubMed

Publication types

Associated data

LinkOut - more resources