Nucleic acid hybridization and the polymerase chain reaction in biology and medicine, with special reference to the detection of human papillomaviruses

Abstract

Nucleic acid hybridization, and more recently, gene amplification by the polymerase chain reaction, have contributed immensely to the development of molecular genetics. These powerful tools have been greeted and employed with great enthusiasm, in view of their wide-ranging applications in biological research and clinical diagnosis. Here we review the basic principles and some applications of these techniques, particularly in infectious diseases and oncogenesis. In order to illustrate their use, data on specific methods as applied to the detection of human papillomaviruses is presented. Gene probe and DNA amplification technology will undoubtedly continue to provide critical molecular pathways in the biomedical research endeavour.