Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Nov;27(11):2619-23.
doi: 10.1128/jcm.27.11.2619-2623.1989.

Serological characterization of bovine rotaviruses isolated from dairy and beef herds in Argentina

R C Bellinzoni  1 , J O Blackhall, N M Mattion, M K Estes, D R Snodgrass, J L LaTorre, E A Scodeller
Affiliations

Serological characterization of bovine rotaviruses isolated from dairy and beef herds in Argentina

R C Bellinzoni et al. J Clin Microbiol. 1989 Nov.

Abstract

Bovine rotaviruses isolated from beef and dairy herds in Argentina were serotyped by the immunoperoxidase focus reduction assay as previously described (G. Gerna, M. Battaglia, G. Milenesi, N. Passarani, E. Percivalle, and E. Cattaneo, Infect. Immun. 43:722-729, 1984). Three strains from beef herds were related to the UK and NCDV bovine rotavirus strains defined as serotype 6 (Y. Hoshino, R. G. Wyatt, H. B. Greenberg, J. Flores, and A. Z. Kapikian, J. Infect. Dis. 149:694-702, 1984). Two other strains from dairy herds were classified as bovine viruses related to the bovine B223 strain reported by Woode and co-workers (G. N. Woode, N. E. Kelso, T. F. Simpson, S. K. Gaul, L. E. Evans, and L. Babiuk, J. Clin. Microbiol. 18:358-364, 1983) in the United States. A serotyping antibody-capture enzyme-linked immunoassay to detect serotype 6 rotavirus using a serotype 6-specific monoclonal antibody was developed and evaluated for strain characterization. Characterization of 72 group A rotavirus-positive fecal samples from beef herds and 43 fecal samples from dairy herds showed a predominance of serotype 6 rotavirus in beef herds but both serotype 6 and non-serotype 6 rotaviruses in dairy herds. Analysis of genomic double-stranded RNA by polyacrylamide gel electrophoresis showed that when outbreaks were caused by one serotype only a single electropherotype was present in all samples.

PubMed Disclaimer

References

    1. Am J Vet Res. 1980 Jan;41(1):151-2 - PubMed
    1. Vaccine. 1989 Jun;7(3):263-8 - PubMed
    1. J Am Vet Med Assoc. 1982 May 15;180(10):1222-6 - PubMed
    1. J Clin Microbiol. 1982 Sep;16(3):495-503 - PubMed
    1. J Clin Microbiol. 1983 Aug;18(2):358-64 - PubMed

Publication types