Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Clinical Trial
. 2015 Apr;21(4):688-95.
doi: 10.1016/j.bbmt.2014.12.017. Epub 2014 Dec 24.

Effect of cord blood processing on transplantation outcomes after single myeloablative umbilical cord blood transplantation

Karen K Ballen  1 , Brent R Logan  2 , Mary J Laughlin  3 , Wensheng He  4 , Daniel R Ambruso  5 , Susan E Armitage  6 , Rachel L Beddard  7 , Deepika Bhatla  8 , William Y K Hwang  9 , Joseph E Kiss  10 , Gesine Koegler  11 , Joanne Kurtzberg  12 , Arnon Nagler  13 , David Oh  14 , Lawrence D Petz  15 , Thomas H Price  16 , Ralph R Quinones  17 , Voravit Ratanatharathorn  18 , J Douglas Rizzo  4 , Kathleen Sazama  19 , Andromachi Scaradavou  20 , Michael W Schuster  21 , Leonard S Sender  22 , Elizabeth J Shpall  6 , Stephen R Spellman  23 , Millicent Sutton  24 , Lee Ann Weitekamp  25 , John R Wingard  26 , Mary Eapen  27
Affiliations
Clinical Trial

Effect of cord blood processing on transplantation outcomes after single myeloablative umbilical cord blood transplantation

Karen K Ballen et al. Biol Blood Marrow Transplant. 2015 Apr.

Abstract

Variations in cord blood manufacturing and administration are common, and the optimal practice is not known. We compared processing and banking practices at 16 public cord blood banks (CBB) in the United States and assessed transplantation outcomes on 530 single umbilical cord blood (UCB) myeloablative transplantations for hematologic malignancies facilitated by these banks. UCB banking practices were separated into 3 mutually exclusive groups based on whether processing was automated or manual, units were plasma and red blood cell reduced, or buffy coat production method or plasma reduced. Compared with the automated processing system for units, the day 28 neutrophil recovery was significantly lower after transplantation of units that were manually processed and plasma reduced (red cell replete) (odds ratio, .19; P = .001) or plasma and red cell reduced (odds ratio, .54; P = .05). Day 100 survival did not differ by CBB. However, day 100 survival was better with units that were thawed with the dextran-albumin wash method compared with the "no wash" or "dilution only" techniques (odds ratio, 1.82; P = .04). In conclusion, CBB processing has no significant effect on early (day 100) survival despite differences in kinetics of neutrophil recovery.

Keywords: Cord blood bank; Processing methods; Survival.

PubMed Disclaimer

Conflict of interest statement

Financial Disclosure Statement: The authors do not have any conflicts to disclose.

Figures

Figure 1
Figure 1
Neutrophil Recovery Group 1: automated processing, units were plasma and red blood cell reduced or buffy coat production method Group 2: manual processing, units were plasma and red blood cell reduced or buffy coat production method Group 3: manual processing, units were plasma reduced
Figure 2
Figure 2
Overall Survival Group 1: automated processing, units were plasma and red blood cell reduced or buffy coat production method Group 2: manual processing, units were plasma and red blood cell reduced or buffy coat production method Group 3: manual processing, units were plasma reduced
See this image and copyright information in PMC

References

    1. Barker JN, Byam CE, Kernan NA, et al. Availability of cord blood extends access to allogeneic hematopoietic stem cell transplant to racial and ethnic minorities. Biol Blood Marrow Transplant. 2010;16:1541–1548. - PMC - PubMed
    1. Chen YB, Aldridge J, Kim HT, et al. Reduced-intensity conditioning stem cell transplantation: comparison of double umbilical cord blood and unrelated donor grafts. Biol Blood Marrow Transplant. 2012;18:805–812. - PubMed
    1. Brunstein CG, Barker JN, Wesidorf DJ, et al. Umbilical cord blood transplantation after nonmyeloablative conditioning: impact on transplantation outcomes in 110 adults with hematologic disease. Blood. 2007;110:3064–3070. - PMC - PubMed
    1. Eapen M, Rocha V, Sanz G, et al. Effect of graft source on unrelated donor hematopoietic stem-cell transplantation in adults with acute leukemia: a retrospective analysis. Lancet Oncology. 2010;11:653–660. - PMC - PubMed
    1. Ballen KK, Spitzer TR, Yeap BY, et al. Double unrelated reduced-intensity umbilical cord blood transplantation in adults. Biol Blood Marrow Transplant. 2007;13:82–89. - PMC - PubMed

Publication types