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. 2015 Mar:94:43-8.
doi: 10.1016/j.plefa.2014.11.003. Epub 2014 Dec 11.

The development of an ELISA for group IVA phospholipase A2 in human red blood cells

Donald J Macdonald  1 , Rose M Boyle  2 , Alastair C A Glen  2 , Christina C Leslie  3 , A Iain M Glen, David F Horrobin
Affiliations

The development of an ELISA for group IVA phospholipase A2 in human red blood cells

Donald J Macdonald et al. Prostaglandins Leukot Essent Fatty Acids. 2015 Mar.

Abstract

An immunoassay for IVA phospholipase A2 in human red blood cells is described. The assay is a non-competitive sandwich assay in which increasing amounts of the measured protein produce increased luminescence. The antibodies used in the assay are directed against two unique epitopes of the molecule, which sequentially trap and detect the protein. The standard curve covers the range 0.7ng to 23ng/mL (0.07 to 2.3ng/well). The intra-assay and inter-assay coefficients of variation were 9% and 12%, respectively. Evidence is presented that the assay is specific for the alpha paralog of IV PLA2. The assay allows simple and rapid quantification of IVAPLA2 in red blood cell lysates and other biological fluids.

Keywords: Alpha paralog; Arachidonic acid; Cell signalling; Diagnostic marker; Red blood cell; Sandwich immunoassay.

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