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. 2015 Feb;12(1):121-3.
doi: 10.1089/zeb.2014.1060. Epub 2014 Dec 30.

Pectoral fin breeding tubercle clusters: a method to determine zebrafish sex

Stephanie C McMillan  1 , Jacqueline Géraudie, Marie-Andrée Akimenko
Affiliations

Pectoral fin breeding tubercle clusters: a method to determine zebrafish sex

Stephanie C McMillan et al. Zebrafish. 2015 Feb.
No abstract available

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Figures

<b>FIG. 1.</b>
FIG. 1.
Distinguishing male and female zebrafish based on large clusters of breeding tubercles (BTs). (A) Male zebrafish possess BT clusters, observed as brown lines, along the central pectoral fin rays (blue arrowheads). (B) Female pectoral fins are translucent, as they do not possess BT clusters (pink arrowhead). (C) Dorsal view of zebrafish in a standard breeding tank. Female pectoral fins (pink arrowheads) can be distinguished from male pectoral fins (blue arrowheads). (D) Under the stereomicroscope, male zebrafish possess BT clusters that appear as spike-like structures along the central fin rays. (F) These structures are absent on female pectoral fins. (E, G) Higher magnification of the central rays of male (E) and female (G) pectoral fins (red boxes in D, F) highlights the presence (blue arrowheads) and lack of BTs, respectively. All scale bars=200 μm. Photographs (A–C) were taken with an 8-megapixel camera on an iPhone 5S. Color images available online at www.liebertpub.com/zeb
See this image and copyright information in PMC

References

    1. McMillan SC, Xu ZT, Zhang J, Teh C, Korzh V, Trudeau VL, et al. . Regeneration of breeding tubercles on zebrafish pectoral fins requires androgens and two waves of revascularization. Development 2013;140:4323–4334 - PubMed
    1. Kang J, Nachtrab G, Poss KD. Local Dkk1 crosstalk from breeding ornaments impedes regeneration of injured male zebrafish fins. Dev Cell 2013;27:19–31 - PMC - PubMed
    1. Fischer B, Metzger M, Richardson R, Knyphausen P, Ramezani T, Franzen R, et al. . P53 and TAp63 Promote keratinocyte proliferation and differentiation in breeding tubercles of the zebrafish. PLoS Genet 2014;10:e1004048. - PMC - PubMed
    1. Géraudie J, Brulfert A, Monnot MJ, Ferretti P. Teratogenic and morphogenetic effects of retinoic acid on the regenerating pectoral fin in zebrafish. J Exp Zool 1994;269:12–22
    1. Avdesh A, Chen M, Martin-Iverson MT, Mondal A, Ong D. Rainey-Smith S., Taddei , et al. . Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction. J Vis Exp 2012;69:e4196. - PMC - PubMed

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