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. 2015 Jul;20(4):163-9.
doi: 10.1179/1351000214Y.0000000121. Epub 2014 Dec 31.

Peripheral mononuclear leukocyte DNA damage, plasma prolidase activity, and oxidative status in patients with benign prostatic hyperplasia

Ilhan Gecit, Ismail Meral, Mehmet Aslan, Abdurrahim Kocyigit, Hakim Celik, Abdullah Taskın, Mehmet Kaba, Necip Pirincci, Mustafa Gunes, Kerem Taken, Halit Demir, Mehmet Uyuklu, Kadir Ceylan

Peripheral mononuclear leukocyte DNA damage, plasma prolidase activity, and oxidative status in patients with benign prostatic hyperplasia

Ilhan Gecit et al. Redox Rep. 2015 Jul.

Abstract

Objectives: Prolidase plays a major role in collagen turnover, matrix remodeling, and cell growth. Benign prostatic hyperplasia (BPH) may be associated with an increased extracellular matrix deposition. Therefore, the present study was designed to investigate the plasma prolidase activity, oxidative status, and peripheral mononuclear leukocyte DNA damage in patients with BPH.

Patients and methods: Twenty-six male patients with BPH and 24 healthy male subjects were included in this study. Blood samples were collected from antecubital vein after an overnight fasting period, and the plasma was separated. Plasma prolidase activity, total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined. The peripheral lymphocyte oxidative DNA damage was determined using an alkaline single cell gel electrophoresis assay (comet assay).

Results: The plasma prolidase activity, TOS levels, OSI values, and peripheral mononuclear leukocyte DNA damage were significantly higher (P < 0.001), while the TAC levels were significantly lower (P < 0.001) in patients with BPH than controls. In BPH patients, the prolidase activity was significantly associated with TAC levels (r = -0.366, P < 0.05), TOS levels (r = 0.573, P < 0.001), and OSI (r = 0.618, P < 0.001) and peripheral mononuclear leukocyte DNA damage (r = 0.461, P < 0.001).

Conclusions: Our results showed that BPH might be associated with an increased oxidative stress, and also an increased plasma prolidase activity. Increased prolidase activity might play an important role in the etiopathogenesis and/or progression of BPH.

Keywords: Benign prostatic hyperplasia; Oxidative stress index; Peripheral leukocyte DNA damage; Prolidase; Total antioxidant capacity; Total oxidant status.

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Conflict of interest statement

Conflict of interest None.

Figures

Figure 1.
Figure 1.
Photomicrographs were showed according to fluorescence intensity in the comet classes (class 0, undamaged; class 4, maximally damaged).
Figure 2.
Figure 2.
Plasma prolidase activity in benign prostatic hyperplasia and healthy subjects.
Figure 3.
Figure 3.
The total antioxidant capacity, total oxidant status, and oxidative stress index in benign prostatic hyperplasia and healthy subjects.
Figure 4.
Figure 4.
Peripheral lymphocyte oxidative DNA damage in benign prostatic hyperplasia and healthy subjects.
See this image and copyright information in PMC

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