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. 2015 Mar;53(3):1002-4.
doi: 10.1128/JCM.03609-14. Epub 2014 Dec 31.

Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR

Sophie Edouard  1 , Elsa Prudent  1 , Philippe Gautret  1 , Ziad A Memish  2 , Didier Raoult  3
Affiliations

Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR

Sophie Edouard et al. J Clin Microbiol. 2015 Mar.

Abstract

We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.

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