Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Dec;63(12):5509-13.
doi: 10.1128/JVI.63.12.5509-5513.1989.

Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins

G Werstuck  1 , J P Capone
Affiliations

Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins

G Werstuck et al. J Virol. 1989 Dec.

Abstract

In order to identify structural domains of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation, subfragments of Vmw65 were expressed in Escherichia coli as fusion polypeptides with protein A of Staphylococcus aureus, and the purified hybrids were used in a band shift assay. The results indicate that a region near the amino terminus of Vmw65 between amino acids 141 and 185 is necessary for complex formation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1987 Aug;61(8):2428-37 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jan;84(1):71-5 - PubMed
    1. Cell. 1988 Feb 12;52(3):425-34 - PubMed
    1. Cell. 1988 Feb 12;52(3):435-45 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6347-51 - PubMed

Publication types

LinkOut - more resources