A comparison of routine light microscopy, immunohistochemistry, and in situ hybridization for the detection of cytomegalovirus in gastrointestinal biopsies
- PMID: 2556912
A comparison of routine light microscopy, immunohistochemistry, and in situ hybridization for the detection of cytomegalovirus in gastrointestinal biopsies
Abstract
Eighty (80) paraffin-embedded intestinal biopsies from 35 AIDS patients were evaluated for the presence of cytomegalovirus by use of biotinylated DNA probe, immunohistochemical assay, and routine hematoxylin and eosin (H&E) staining. Cytomegalovirus was detected in 13 biopsies (16.3%) from five patients (14.2%). The distribution of positive biopsies was one in seven esophageal biopsies, three in 19 small bowel biopsies, six in 27 colonic biopsies, and three in 17 rectal biopsies. Immunoelectron microscopy was employed to confirm the presence of CMA in four of the positive in situ hybridization cases. In situ hybridization for CMV DNA was more sensitive than immunostaining, and both proved superior to routine H&E staining in terms of sensitivity.
Similar articles
-
Poor diagnostic value of in situ hybridization and immunohistochemistry in endomyocardial biopsies to detect cytomegalovirus after heart transplantation.J Heart Lung Transplant. 1992 Jul-Aug;11(4 Pt 1):773-7. J Heart Lung Transplant. 1992. PMID: 1323328
-
Correlation of in situ hybridization with histology and viral culture in patients with acquired immunodeficiency syndrome with cytomegalovirus colitis.Arch Pathol Lab Med. 1989 Oct;113(10):1124-6. Arch Pathol Lab Med. 1989. PMID: 2552953
-
In situ DNA hybridization for cytomegalovirus in colonoscopic biopsies.Arch Pathol Lab Med. 1988 Nov;112(11):1106-9. Arch Pathol Lab Med. 1988. PMID: 2845884
-
Detection and quantification of human cytomegalovirus (CMV) as a marker for development of CMV disease and survival in patients with AIDS.Antivir Ther. 1997 Dec;2(4):200-5. Antivir Ther. 1997. PMID: 11327439 Review. No abstract available.
-
The clinicopathological significance of cytomegalovirus inclusions demonstrated by endocervical biopsy.Pathology. 1991 Oct;23(4):318-21. doi: 10.3109/00313029109063596. Pathology. 1991. PMID: 1664515 Review.
Cited by
-
Diagnostic value of amplification of human cytomegalovirus DNA from gastrointestinal biopsies from human immunodeficiency virus-infected patients.J Clin Microbiol. 1993 Aug;31(8):2066-9. doi: 10.1128/jcm.31.8.2066-2069.1993. J Clin Microbiol. 1993. PMID: 8396587 Free PMC article.
-
Evidence-based consensus on opportunistic infections in inflammatory bowel disease (republication).Intest Res. 2018 Apr;16(2):178-193. doi: 10.5217/ir.2018.16.2.178. Epub 2018 Apr 30. Intest Res. 2018. PMID: 29743831 Free PMC article. Review.
-
Cytomegalovirus-induced Hemorrhagic Colitis in a Patient with Chronic Myeloid Leukemia (Chronic Phase) on Dasatinib as an Upfront Therapy.Clin Med Insights Case Rep. 2015 Aug 26;8:77-81. doi: 10.4137/CCRep.S25327. eCollection 2015. Clin Med Insights Case Rep. 2015. PMID: 26379451 Free PMC article.
-
Comparison of different methods for CMV detection.Int J Legal Med. 1994;106(4):219-22. doi: 10.1007/BF01371341. Int J Legal Med. 1994. PMID: 8038116
-
Systematic review: cytomegalovirus infection in inflammatory bowel disease.J Gastroenterol. 2008;43(10):735-40. doi: 10.1007/s00535-008-2246-x. Epub 2008 Oct 29. J Gastroenterol. 2008. PMID: 18958541 No abstract available.