Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Dec;109(6 Pt 2):3419-24.
doi: 10.1083/jcb.109.6.3419.

Transforming growth factors-beta 1 and beta 2 are mitogens for rat Schwann cells

A J Ridley  1 , J B Davis, P Stroobant, H Land
Affiliations

Transforming growth factors-beta 1 and beta 2 are mitogens for rat Schwann cells

A J Ridley et al. J Cell Biol. 1989 Dec.

Abstract

Transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 2 were found to be potent mitogens for purified rat Schwann cells, each stimulating DNA synthesis in quiescent cells and also increasing their proliferation rate. Half-maximal stimulation of DNA synthesis occurred at approximately 0.1 ng/ml TGF-beta 1 or TGF-beta 2. Mitogenic stimulation by TGF-beta 1 and TGF-beta 2 was enhanced by forskolin, which activates adenylate cyclase, at concentrations up to 0.5 microM forskolin. However, at 5 microM forskolin, the synergistic interaction between forskolin and TGF-beta 1 was abolished. These results are in contrast to the observed synergy between forskolin and another Schwann cell mitogen, glial growth factor (GGF). Both 0.5 and 5 microM forskolin were found to enhance the stimulation of DNA synthesis by partially purified GGF (GGF-CM). As well as being functionally distinct, TGF-beta 1 and GGF-CM activities were also physically separable by chromatography on a Superose 12 gel permeation column. Thus, TGF-beta 1 and beta 2 are rat Schwann cell mitogens, and Schwann cells are one of the few normal cell populations to respond mitogenically to TGF-beta.

PubMed Disclaimer

References

    1. J Cell Physiol. 1984 Oct;121(1):184-8 - PubMed
    1. Nature. 1984 Aug 23-29;310(5979):655-60 - PubMed
    1. Nature. 1985 Aug 22-28;316(6030):701-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2453-7 - PubMed
    1. J Neurosci. 1986 Oct;6(10):3070-8 - PubMed