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. 2014:2014:274679.
doi: 10.1155/2014/274679. Epub 2014 Dec 9.

Detection of influenza virus infection using two PCR methods

Richard K Zimmerman  1 , Charles R Rinaldo  2 , Mary Patricia Nowalk  1 , G K Balasubramani  3 , Mark G Thompson  4 , Arlene Bullotta  5 , Michael Susick  1 , Stephen Wisniewski  3
Affiliations

Detection of influenza virus infection using two PCR methods

Richard K Zimmerman et al. Adv Virol. 2014.

Abstract

Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses.

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