Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Nov 12;5(12):4242-8.
doi: 10.1364/BOE.5.004242. eCollection 2014 Dec 1.

Simultaneous optical coherence tomography and lipofuscin autofluorescence imaging of the retina with a single broadband light source at 480nm

Minshan Jiang  1 , Tan Liu  2 , Xiaojing Liu  2 , Shuliang Jiao  2
Affiliations

Simultaneous optical coherence tomography and lipofuscin autofluorescence imaging of the retina with a single broadband light source at 480nm

Minshan Jiang et al. Biomed Opt Express. .

Abstract

We accomplished spectral domain optical coherence tomography and auto-fluorescence microscopy for imaging the retina with a single broadband light source centered at 480 nm. This technique is able to provide simultaneous structural imaging and lipofuscin molecular contrast of the retina. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. To test the capabilities of the technique we periodically imaged the retinas of the same rats for four weeks. The images successfully demonstrated lipofuscin accumulation in the retinal pigment epithelium with aging. The experimental results showed that the dual-modal imaging system can be a potentially powerful tool in the study of age-related degenerative retinal diseases.

Keywords: (110.4500) Optical coherence tomography; (170.2520) Fluorescence microscopy.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
(a) Schematic of the experimental system. C1-C3: Collimator; L1-L3: Lens; DM: Dichroic mirror; LPF: Long-pass filter; PC: Polarization controller; PMT: Photomultiplier; (b) The measured spectrum of selected output from the light source.
Fig. 2
Fig. 2
VIS-OCT cross-sectional image of a rat retina consisting of 2048 depth scans. Bar: 200 μm.
Fig. 3
Fig. 3
VIS-OCT and AF images simultaneously acquired from a rat retina in vivo. (a) OCT fundus image; (b) AF image; (c) OCT B-scan image. Bar: 200 μm. The white line in the OCT fundus image marks the location of the OCT B-scan image.
Fig. 4
Fig. 4
OCT fundus images (a, d, g), AF images (b, e, h) and histograms of the AF intensity distributions (c, f, i) of one rat at different ages: (a) – (c) 10 weeks, (d) – (f) 12 weeks, (g)-(i) 14 weeks. Numbers 1, 2 and 3 mark the corresponding vessels at different time point.
Fig. 5
Fig. 5
The mean AF intensity plus standard deviation of the 3 rats at different ages.
See this image and copyright information in PMC

References

    1. Huang D., Swanson E. A., Lin C. P., Schuman J. S., Stinson W. G., Chang W., Hee M. R., Flotte T., Gregory K., Puliafito C. A., “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).10.1126/science.1957169 - DOI - PMC - PubMed
    1. Fercher A. F., “Optical coherence tomography,” J. Biomed. Opt. 1(2), 157–173 (1996).10.1117/12.231361 - DOI - PubMed
    1. Ramanujam N., “Fluorescence spectroscopy of neoplastic and non-neoplastic tissues,” Neoplasia 2(1-2), 89–117 (2000).10.1038/sj.neo.7900077 - DOI - PMC - PubMed
    1. Denk W., Strickler J. H., Webb W. W., “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).10.1126/science.2321027 - DOI - PubMed
    1. Gray D. C., Merigan W., Wolfing J. I., Gee B. P., Porter J., Dubra A., Twietmeyer T. H., Ahamd K., Tumbar R., Reinholz F., Williams D. R., “In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells,” Opt. Express 14(16), 7144–7158 (2006).10.1364/OE.14.007144 - DOI - PubMed