Elevated CO₂ mitigates drought and temperature-induced oxidative stress differently in grasses and legumes

Abstract

Increasing atmospheric CO2 will affect plant growth, including mitigation of stress impact. Such effects vary considerably between species-groups. Grasses (Lolium perenne, Poa pratensis) and legumes (Medicago lupulina, Lotus corniculatus) were subjected to drought, elevated temperature and elevated CO2. Drought inhibited plant growth, photosynthesis and stomatal conductance, and induced osmolytes and antioxidants in all species. In contrast, oxidative damage was more strongly induced in the legumes than in the grasses. Warming generally exacerbated drought effects, whereas elevated CO2 reduced stress impact. In the grasses, photosynthesis and chlorophyll levels were more protected by CO2 than in the legumes. Oxidative stress parameters (lipid peroxidation, H2O2 levels), on the other hand, were generally more reduced in the legumes. This is consistent with changes in molecular antioxidants, which were reduced by elevated CO2 in the grasses, but not in the legumes. Antioxidant enzymes decreased similarly in both species-groups. The ascorbate-glutathione cycle was little affected by drought and CO2. Overall, elevated CO2 reduced drought effects in grasses and legumes, and this mitigation was stronger in the legumes. This is possibly explained by stronger reduction in H2O2 generation (photorespiration and NADPH oxidase), and a higher availability of molecular antioxidants. The grass/legume-specificity was supported by principal component analysis.

Keywords: Elevated CO(2); Global change; Grassland species; Oxidative stress; Plant redox network; Water deficit stress.