A generic and rapid strategy for determining trace multiresidues of sulfonamides in aquatic products by using an improved QuEChERS method and liquid chromatography-electrospray quadrupole tandem mass spectrometry

Abstract

A rapid and efficient multiresidue method that involves using improved QuEChERS method and liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) was developed to measure trace levels of sulfonamides in fish tissue. This proposed method was proven to be a powerful, highly sensitive, and environmentally friendly analytical tool that requires minimal sample preparation. The typical MS/MS fragmentation patterns of the [M+H](+) were 156 m/z, 108 m/z, and 92 m/z. Separation was performed on HC-C18 columns with a gradient elution by using methanol -5mM ammonium acetate containing formic acid (pH 3.5). This method was validated and exhibited favorable performance as well as acceptable accuracy (80.2-93.5%), precision (3.82-8.71%), sensitivity (limits of detection (LODs) 0.43-1.22 μg kg(-1), limits of quantification (LOQs) 1.27-3.71 μg kg(-1); decision limit (CCα) 1.49-10.9 μg kg(-1), detection capability (CCβ) 1.71-11.4 μg kg(-1)), and an acceptable matrix effect (-18.2-18.4%). This methodology has been successfully applied in analyzing various fish tissue from local markets.

Keywords: Dispersive solid-phase extraction; Fish tissue; LC–ESI–MS/MS; QuEChERS; Sulfachloropyridazine (PubChem CID: 6634); Sulfadiazine (PubChem CID: 5215); Sulfadimethoxine (PubChem CID: 5323); Sulfadoxine (PubChem CID: 17134); Sulfamethizole (PubChem CID: 5328); Sulfamethoxazole (PubChem CID: 5329); Sulfamethoxypyridazine (PubChem CID: 5330); Sulfamonomethoxine (PubChem CID: 5332); Sulfaquinoxaline (PubChem CID: 5338); Sulfathiazole (PubChem CID: 5340); Sulfonamides.