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. 2014:2014:496562.
doi: 10.1155/2014/496562. Epub 2014 Dec 15.

Specific genomic fingerprints of phosphate solubilizing Pseudomonas strains generated by BOX elements

Mohammad Bagher Javadi Nobandegani  1 , Halimi Mohd Saud  1 , Wong Mui Yun  1
Affiliations

Specific genomic fingerprints of phosphate solubilizing Pseudomonas strains generated by BOX elements

Mohammad Bagher Javadi Nobandegani et al. Biomed Res Int. 2014.

Abstract

Primers corresponding to conserved bacterial repetitive of BOX elements were used to show that BOX-DNA sequences are widely distributed in phosphate solubilizing Pseudomonas strains. Phosphate solubilizing Pseudomonas was isolated from oil palm fields (tropical soil) in Malaysia. BOX elements were used to generate genomic fingerprints of a variety of Pseudomonas isolates to identify strains that were not distinguishable by other classification methods. BOX-PCR, that derived genomic fingerprints, was generated from whole purified genomic DNA by liquid culture of phosphate solubilizing Pseudomonas. BOX-PCR generated the phosphate solubilizing Pseudomonas specific fingerprints to identify the relationship between these strains. This suggests that distribution of BOX elements' sequences in phosphate solubilizing Pseudomonas strains is the mirror image of their genomic structure. Therefore, this method appears to be a rapid, simple, and reproducible method to identify and classify phosphate solubilizing Pseudomonas strains and it may be useful tool for fast identification of potential biofertilizer strains.

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Figures

Figure 1
Figure 1
PCR products of 16S rDNA (a) and BOX element (b) (Line 1 = 18DNR, Line 2 = 5DNR, Line 3 = 41DNR, Line 4 = 22DNR, Line 5 = 31SR, and Line 6 = 8SR).
Figure 2
Figure 2
Dendrogram of phosphate solubilizing Pseudomonas bacteria based on the 16S rDNA (a) and BOX-PCR marker (b).
See this image and copyright information in PMC

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