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. 2014 Nov;8(11):ZC31-4.
doi: 10.7860/JCDR/2014/9876.5125. Epub 2014 Nov 20.

Detection of rankl positive cells in gingival tissue in healthy & chronic periodontal disease patients-a comparative study

J Bhuvaneswarri  1 , Bagavad Gita  2 , S C Chandrasekaran  3
Affiliations

Detection of rankl positive cells in gingival tissue in healthy & chronic periodontal disease patients-a comparative study

J Bhuvaneswarri et al. J Clin Diagn Res. 2014 Nov.

Abstract

Aim & Objective: The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins implicated in osteoclastogenesis. This study aimed to identify & quantify RANKL positive cells in gingival tissues in healthy & diseased patients & the study looks for immunohistochemical evidence of the expression of the protein in gingival tissue samples.

Materials and methods: Patients were randomly selected. Thirty subject each for (test & control). Age range of 25-55y, either sex was selected. Tissue samples were collected from Control - Healthy Gingiva & Test-Chronic Periodontitis Patients. Tissue sections were prepared. An Immunohistochemical analysis was done & cell counting was done for RANKL positive cells.

Results: Immunohistochemical staining showed that RANKL-positive cells were significantly distributed in the inflammatory epithelium & connective tissue zone of diseased & non-diseased gingiva. RANKL, positive cells was increased significantly in patients with chronic periodontitis (p < 0.05).

Conclusion: These findings imply that in this comparative study of gingival tissue for, RANKL positive cells, these cells were present in both healthy & chronic periodontitis samples, but number of positive cells present is significantly increased in chronic periodontitis.

Keywords: Chronic periodontitis; Osteoprotegrin; RANKL.

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Figures

[Table/Fig-3]:
[Table/Fig-3]:
Total number of the receptor activator of nuclear factor-kappa B ligand (RANKL) positive cells in gingival tissue obtained from either sex
[Table/Fig-7]:
[Table/Fig-7]:
Strong cytoplasmic staining of RANKL positive cells in diseased tissues. (40X microscopic Image)
[Table/Fig-8]:
[Table/Fig-8]:
Shows RANKL positive cells in healthy tissue. (40X microscopic Image)
See this image and copyright information in PMC

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