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. 2014:2014:510157.
doi: 10.1155/2014/510157. Epub 2014 Dec 18.

Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo

Enhui Cui  1 , Xiaoyan Zhi  1 , Ying Chen  1 , Yuanyuan Gao  1 , Yunpeng Fan  1 , Weimin Zhang  1 , Wuren Ma  1 , Weifeng Hou  1 , Chao Guo  1 , Xiaoping Song  1
Affiliations

Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo

Enhui Cui et al. Evid Based Complement Alternat Med. 2014.

Abstract

Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced by xylene and formaldehyde, respectively. In vitro, cytotoxicity, the phagocytosis of macrophages, the levels of nitric oxide (NO), induced nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in cell supernatant were detected. In vivo, swelling rate and edema inhibitory rate of ear and paw were observed using CM-treated mice. Results. At 150-18.75 μg·mL(-1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS. In vivo, pretreatment with CM, the ear swelling, and paw edema of mice could be significantly inhibited in a dose-dependent manner, and the antiedema effect of CM at high dose was better than dexamethasone. Conclusion. Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.

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Figures

Figure 1
Figure 1
Chromatogram of berberine obtained at 345 nm. (a) Berberine standard; (b) the extract of CM.
Figure 2
Figure 2
The cell viability of macrophages incubated with CM. a-gBars without the same superscripts differ significantly (P < 0.05).
Figure 3
Figure 3
The effect of CM on phagocytosis of macrophages. a-dBars without the same superscripts differ significantly (P < 0.05).
Figure 4
Figure 4
Effect of CM on the production of NO. a-cBars without the same superscripts differ significantly (P < 0.05).
Figure 5
Figure 5
Effect of CM on the production of iNOS. a-cBars without the same superscripts differ significantly (P < 0.05).
Figure 6
Figure 6
Effect of CM on the TNF-α secretion. a-dBars without the same superscripts differ significantly (P < 0.05).
Figure 7
Figure 7
Effect of CM on the IL-6 secretion. a-cBars without the same superscripts differ significantly (P < 0.05).
Figure 8
Figure 8
Effect of CM on xylene induced ear swelling in mice. a-bBars without the same superscripts differ significantly (P < 0.05). H: high dose; M: medium dose; L: low dose; BC: blank control.
Figure 9
Figure 9
Effect of CM on formaldehyde induced paw edema in mice. a-bBars without the same superscripts differ significantly (P < 0.05). H: high dose; M: medium dose; L: low dose; BC: blank control.
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