Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Winter;4(1):43-7.

Foll Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia

Mousa Tavassoli  1 , Mohammad Ghorbanzadehghan  2 , Bijan Esmaeilnejad  1
Affiliations

Foll Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia

Mousa Tavassoli et al. Vet Res Forum. 2013 Winter.

Abstract

Infection by the protozoan parasite Toxoplasma gondii, is widespread in humans and many other warm-blooded animals. More than half billion of world human population has serum antibodies to T. gondii and Sheep and goats are more widely infected with T. gondii. T. gondii infection can be diagnosed indirectly with serological methods and directly by polymerase chain reaction (PCR), hybridization, isolation and histology. A total number of 124 goats and 113 sheep blood samples were collected from Urmia region and PCR was used for detection of the pathogenic protozoan T. gondii using B1 gene. The targeted B1 gene is highly conserved in all T. gondii strains and is multiple copy genes whit in the T. gondii genome. The method used for the characterization of T. gondii strains implied digestion with AluI restriction enzyme of the fragments amplified. The results indicated three positive sheep (1.26%) with one RFLP patterns. The results indicated that the same strain of T. gondii has infected sheep in the region.

Keywords: Goat; PCR-RFLP; Sheep; Toxoplasma gondii; Urmia.

PubMed Disclaimer

Figures

Fig 1
Fig 1
PCR product (529 bp) of B1 gene amplified from infected sheep with T. gondii: Lane 1: 50 bp marker, Lane 2: positive control, Lane 3: negative control, Lane 4-6: positive samples
Fig 2
Fig 2
PCR-RFLP pattern of B1 gene amplified from infected sheep with T. gondii: Lane 1: 50 bp marker, Lane 2: PCR product, Lane 3-5: RFLP pattern.
See this image and copyright information in PMC

References

    1. Dubey JP, Beattie C. Toxoplasmosis of animal and man. Boca Raton: CRC Press ; 1988. p. 220.
    1. Asthana SP, Macpherson CNL, Weiss SH, et al. Sero-prevalence of Toxoplasma gondii in pregnant women and cats in Grenada. West Indies J Parasitol. 2006;92:644–645. - PubMed
    1. Abdi J, Shojaee S, Mirzaee A, et al. Seroprevalance of toxoplasmosis in pregnant women in Ilam proviance, Iran. Iranian J Parasitol. 2008;3(2):34–37.
    1. Jalayer T, Allameh T. Seroprevalance of congenital toxoplasmosis in neonatal born in Isfahan. The 2nd congress of parasitology; Tehran, Iran : 1997. p. 23.
    1. Moalaee H, Shirzad E, Namazi MJ. Seroepidemiology of toxoplasmosis and its eye complication in pregnant women. Sabzevar University Med Sci J. 1999:21–23.

LinkOut - more resources