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. 2013 Winter;4(1):55-8.

Detection of torque teno virus (TTV) in domestic village chickens in Iran

Majid Bouzari  1 , Nima Shaykh Baygloo  1
Affiliations

Detection of torque teno virus (TTV) in domestic village chickens in Iran

Majid Bouzari et al. Vet Res Forum. 2013 Winter.

Abstract

Torque teno virus (TTV) is prevalent worldwide and has been extensively studied in human and some wild and domestic animals. As the studies on TTV in chickens was rare and there was no information about the infection of domestic village chickens with TTV and also structural resemblance of this virus to chicken anemia virus, the frequency of the infection in domestic village chickens in different villages in Isfahan (Iran) was investigated. Sera were collected from 50 chickens. Viral DNA was extracted and subjected to polymerase chain reaction (PCR) using the previously described T801 and T935 primers that were used for amplification of a highly conserved non-coding region (UTR) of the viral genome in a single round of PCR and Set B primers of conserved region in a nested PCR reaction. Using T801 and T835 primers TTV or viruses of TTV family were detected in 16 out of 50 sera tested (32%). Fourteen out of the same 50 sera (28%) were positive for TTV using Set B primers. Totally 20 sera were positive using both primers (40%). Ten sera were detected with both sets of primers, six sera with T801 and T935 primers and only four sera were positive using Set B primers for TTV. Different patterns of the detection of the virus with the two different sets of primers suggests the possibility of the presence of different genotypes of TTV in domestic village chickens and the possibility of the transmission of the virus from human to village chickens and vice versa. This necessitates further investigations.

Keywords: Domestic village chickens; Iran; PCR; Torque Teno Virus (TTV).

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Figures

Fig. 1
Fig. 1
Gel electrophoresis results of single round PCR with the T801 and T935 primers. M = 100 bp marker (Fermentas, St. Leon-Rot, Germany); 1 and 3 = positive samples; 2 = negative sample; P = positive control and N = negative control
Fig. 2
Fig. 2
Gel electrophoresis results of the nested PCR with the Set B primers. M = 100 bp marker (Fermentas, St. Leon-Rot, Germany); 1, 2, 3, 4 and 5 = positive samples; P = positive control and N = negative control
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