Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Apr;53(4):1351-4.
doi: 10.1128/JCM.03412-14. Epub 2015 Jan 21.

Discordance between Xpert MTB/RIF assay and Bactec MGIT 960 Culture System for detection of rifampin-resistant Mycobacterium tuberculosis isolates in a country with a low tuberculosis (TB) incidence

Eiman Mokaddas  1 , Suhail Ahmad  2 , Hanaa S Eldeen  3 , Noura Al-Mutairi  4
Affiliations

Discordance between Xpert MTB/RIF assay and Bactec MGIT 960 Culture System for detection of rifampin-resistant Mycobacterium tuberculosis isolates in a country with a low tuberculosis (TB) incidence

Eiman Mokaddas et al. J Clin Microbiol. 2015 Apr.

Abstract

Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpert assay. rpoB sequencing identified a silent (CTG521TTG) mutation in one isolate and a missense (GAC516TAC) mutation in another. The detection of rifampin resistance is imperfect with both the Xpert assay and MGIT system. Any discordant rifampin resistance results should be confirmed by sequencing of the rpoB gene.

PubMed Disclaimer

References

    1. World Health Organization. 2014. Global tuberculosis report 2014. WHO/HTM/TB/2014.08. World Health Organization, Geneva, Switzerland. 2014: http://apps.who.int/iris/bitstream/10665/137094/1/9789241564809_eng.pdf?....
    1. Ahmad S, Mokaddas E. 2014. Current status and future trends in the diagnosis and treatment of drug-susceptible and multidrug-resistant tuberculosis. J Infect Public Health 7:75–91. doi:10.1016/j.jiph.2013.09.001. - DOI - PubMed
    1. Drobniewski F, Nikolayevskyy V, Balabanova Y, Bang D, Papaventsis D. 2012. Diagnosis of tuberculosis and drug resistance: what can new tools bring us? Int J Tuberc Lung Dis 16:860–870. doi:10.5588/ijtld.12.0180. - DOI - PubMed
    1. Telenti A, Imboden P, Marchesi F, Lowrie D, Cole S, Colston MJ, Matter L, Schopfer K, Bodmer T. 1993. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet 341:647–650. doi:10.1016/0140-6736(93)90417-F. - DOI - PubMed
    1. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, Allen J, Tahirli R, Blakemore R, Rustomjee R, Milovic A, Jones M, O'Brien SM, Persing DH, Ruesch-Gerdes S, Gotuzzo E, Rodrigues C, Alland D, Perkins MD. 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med 363:1005–1015. doi:10.1056/NEJMoa0907847. - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources