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. 1989 Oct;7(9):940-2.

Construction of cloning/sequencing vectors with an alternative polylinker

L A Howard  1 , S A Ortlepp
Affiliations
  • PMID: 2561063

Construction of cloning/sequencing vectors with an alternative polylinker

L A Howard et al. Biotechniques. 1989 Oct.

Abstract

This paper describes the construction of a new plasmid and M13 phage cloning vector in which the 54-bp polylinkers of pUC19 and of M13mp8 are replaced with a 45-bp chemically synthesized polylinker with different restriction sites. The new polylinker is inserted in frame at the N-terminal end of the beta galactosidase lac Z fragment. The plasmid was named pLH1, the phage M13LH1.

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