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. 2014:2014:815495.
doi: 10.1155/2014/815495. Epub 2014 Dec 30.

Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

K Y Usha  1 , K Praveen  2 , B Rajasekhar Reddy  1
Affiliations

Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

K Y Usha et al. Biotechnol Res Int. 2014.

Abstract

The white rot fungi Stereum ostrea displayed a wide diversity in their response to supplemented inducers, surfactants, and copper sulphate in solid state fermentation. Among the inducers tested, 0.02% veratryl alcohol increased the ligninolytic enzyme production to a significant extent. The addition of copper sulphate at 300 μM concentration has a positive effect on laccase production increasing its activity by 2 times compared to control. Among the surfactants, Tween 20, Tween 80, and Triton X 100, tested in the studies, Tween 80 stimulated the production of ligninolytic enzymes. Biosorption of dyes was carried out by using two lignocellulosic wastes, rice bran and wheat bran, in 50 ppm of remazol brilliant blue and remazol brilliant violet 5R dyes. These dye adsorbed lignocelluloses were then utilized for the production of ligninolytic enzymes in solid state mode. The two dye adsorbed lignocelluloses enhanced the production of laccase and manganese peroxidase but not lignin peroxidase.

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Figures

Figure 1
Figure 1
Effect of inducers on secretion of extracellular proteins. A: gallic acid, B: tannic acid, C: guaiacol, D: veratryl alcohol, E: lignin, F: lignosulfonic acid, and G: control. Values are the means of duplicates. Means, in each column, followed by same letter are not significantly different (P ≤ 0.05) from each other according to DMR test.
Figure 2
Figure 2
Influence of copper sulphate on laccase production. Values are the means of duplicates. Means, in each column, followed by same letter are not significantly different (P ≤ 0.05) from each other according to DMR test.
Figure 3
Figure 3
Effect of copper sulphate on secretion of extracellular proteins. Values are the means of duplicates. Means, in each column, followed by same letter are not significantly different (P ≤ 0.05) from each other according to DMR test.
Figure 4
Figure 4
Effect of surfactants on secretion of extracellular protein. Values are the means of duplicates. Means, in each column, followed by same letter are not significantly different (P ≤ 0.05) from each other according to DMR test.
Figure 5
Figure 5
Secretion of extracellular proteins by dye adsorbed lignocelluloses. RBB-RB: remazol brilliant blue adsorbed rice bran, RBB-WB: remazol brilliant blue adsorbed wheat bran, RBV-WB: remazol brilliant violet 5R adsorbed wheat bran, and RBV-RB: remazol brilliant violet 5R adsorbed rice bran. Values are the means of duplicates. Means, in each column, followed by same letter are not significantly different (P ≤ 0.05) from each other according to DMR test.
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