Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jan;25(1):79-85.
doi: 10.1007/s10895-014-1482-0. Epub 2015 Jan 25.

Rapid fluorescence spectroscopic characterization of salivary DNA of normal subjects and OSCC patients using ethidium bromide

Manoharan Yuvaraj  1 , Prakasarao Aruna, Dornadula Koteeswaran, Palraj Tamilkumar, Singaravelu Ganesan
Affiliations

Rapid fluorescence spectroscopic characterization of salivary DNA of normal subjects and OSCC patients using ethidium bromide

Manoharan Yuvaraj et al. J Fluoresc. 2015 Jan.

Abstract

Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited state kinetics of salivary DNA has been performed with 27 normal subjects and 67 OSCC patients saliva using ethidium bromide as a probe to look for the possibility in discrimination between them. On statistical analysis the sensitivity and specificity of 88.9 and 94.0 % has been achieved from the fluorescence emission spectra and 88.9 and 92.5 % with that of fluorescence excitation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. FASEB J. 2009 Nov;23 (11):3790-807 - PubMed
    1. Proc Natl Acad Sci U S A. 1970 Feb;65(2):417-21 - PubMed
    1. J Photochem Photobiol B. 2014 Jan 5;130:153-60 - PubMed
    1. Photochem Photobiol. 2003 Aug;78(2):197-204 - PubMed
    1. Expert Rev Mol Med. 2002 Apr 11;4(9):1-19 - PubMed

Publication types

LinkOut - more resources