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. 2015 Feb;9(2):915-919.
doi: 10.3892/ol.2014.2768. Epub 2014 Dec 4.

Expression levels of matrix metalloproteinase-9 in human gastric carcinoma

Su-Zuan Chen  1 , Huai-Qi Yao  1 , Sen-Zhi Zhu  1 , Qiu-Yuan Li  1 , Guang-Hua Guo  1 , Jing Yu  1
Affiliations

Expression levels of matrix metalloproteinase-9 in human gastric carcinoma

Su-Zuan Chen et al. Oncol Lett. 2015 Feb.

Abstract

The present report investigated the correlation between the expression levels of matrix metalloproteinase (MMP)-9 in gastric carcinoma patients and the clinicopathological characteristics. Forty-five samples of gastric carcinoma and distal gastric mucosa tissue, and 10 samples of healthy gastric mucosa tissue were analyzed using semi-quantitative polymerase chain reaction, as well as immunohistochemical and hematoxylin and eosin staining. MMP-9 protein levels in serum samples from the same patients were quantified by enzyme-linked immunosorbent assay. The present report identified that MMP-9 expression was markedly higher in the gastric carcinoma tissue (86.67%) than in the adjacent healthy tissue (10.00%). A positive association was identified between the level of MMP-9 protein expression and the depth of cancer invasion (P<0.05). Furthermore, the preoperative serum levels of the MMP-9 protein in the gastric carcinoma tissue were correlated with the tumor-node-metastasis stage and occurrence of lymph node metastasis (P<0.01). Data from the present report indicates that MMP-9 may be key in gastric carcinoma malignancy, and implies that MMP-9 may serve as a novel biomarker in the diagnosis and prognosis of gastric carcinoma.

Keywords: gastric carcinoma; immunohistochemistry; malignancy; matrix metalloproteinases-9; semi-quantitative polymerase chain reaction.

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Figures

Figure 1
Figure 1
mRNA expression of MMP-9 and β-actin extracted from gastric carcinoma tissue (ca), distal gastric mucosa tissue (n1) and healthy gastric tissue (n2). MMP-9, matrix metalloproteinase-9.
Figure 2
Figure 2
Hematoxylin and eosin staining of matrix metalloproteinase-9 in gastric carcinoma tissue, as shown by light gray grains in the cytoplasm (magnification, ×200).
Figure 3
Figure 3
Immunohistochemical staining of matrix metalloproteinase-9 in gastric carcinoma tissue, as shown by dark grains in the cytoplasm (magnification, ×400).
See this image and copyright information in PMC

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