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. 2015 Mar;67(Suppl 1):47-50.
doi: 10.1007/s12070-014-0747-x. Epub 2014 Aug 2.

Utility of toluidine blue test in accessing and detecting intra-oral malignancies

Deepti Singh  1 , R K Shukla  2
Affiliations

Utility of toluidine blue test in accessing and detecting intra-oral malignancies

Deepti Singh et al. Indian J Otolaryngol Head Neck Surg. 2015 Mar.

Abstract

In vivo staining reveals cytological details that might otherwise not be apparent. The aim of the study was to test the utility of toluidine blue test in detecting various types of malignant and premalignant lesions in early stage. Fifty patients with lesion in oral cavity having suspicion of malignancy clinically were selected. After subjecting the patients to clinical examination, the suspicious lesions were stained with 1 % toluidine blue. The biopsy site was selected on the basis of clinical appearance and dye retention and in the sites where no retention of the stain occurred, clinical judgment directed the biopsy site. The sensitivity of toluidine blue in detecting premalignant or malignant lesions was found to be 97.8 % and the over all specificity was found to be 100 %. The positive predictive value, negative predictive value and diagnostic accuracy was reported to be 100, 80 and 90 % respectively. Toluidine Blue staining is highly a reliable source for the detection of insitu and invasive carcinomas. Staining with this stains is an adjunct to clinical judgment, assist in the choice of biopsy site, follow up of premalignant lesions and marginal demarcation of the malignant lesions enabling an intervention method to be adopted earlier for the disease, which carries a high rate of morbidity and mortality.

Keywords: Early detection; In vivo staining; Intra-oral malignancies; Toluidine blue.

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Figures

Fig. 1
Fig. 1
Ulcerative lesion tongue stained with toluidine
Fig. 2
Fig. 2
Lesion rt. Buccal mucosa stained with toluidine
Fig. 3
Fig. 3
Presenting complaints of patients
Fig. 4
Fig. 4
Addiction present in patients
Fig. 5
Fig. 5
Result of toluidine blue staining and histopathology
See this image and copyright information in PMC

References

    1. Rosenberg D, Cretin S. Use of meta-analysis to evaluate tolonium chloride in oral cancer screening. Oral Surg Oral Med Oral Pathol. 1989;67:621–627. doi: 10.1016/0030-4220(89)90286-7. - DOI - PubMed
    1. Epstein JB, Scully C, Spinelli JJ. Toluidine blue and Lugol’s iodine application in the assessment of oral malignant disease and lesions at risk of malignancy. J Oral Pathol Med. 1992;21:160–163. doi: 10.1111/j.1600-0714.1992.tb00094.x. - DOI - PubMed
    1. Yokoo K, Noma H, Inoue T, Hashimoto S, Shimono M. Cell proliferation and tumour suppressor gene expression in iodine-unstained area surrounding oral squamous cell carcinoma. Int J Oral Maxillofacial Surg. 2004;33:75–83. doi: 10.1054/ijom.2002.0457. - DOI - PubMed
    1. Mashberg A. Re-evaluation of toluidine blue application as a diagnostic adjunct in the detection of asymptomatic oral squamous carcinoma. Cancer. 1980;46:758–763. doi: 10.1002/1097-0142(19800815)46:4<758::AID-CNCR2820460420>3.0.CO;2-8. - DOI - PubMed
    1. Mashberg A. Tolonium (toluidine blue) rinse a screening method for recognition of squamous carcinoma—a continuing study of Oral Cancer IV. JAMA. 1981;245:2408–2410. doi: 10.1001/jama.1981.03310480024019. - DOI - PubMed

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