Neuroglobin up-regulation after ischaemic pre-conditioning in a rat model of middle cerebral artery occlusion

Abstract

Primary objective: Neuroglobin (NGB) is a known neuroprotector and is up-regulated after ischaemia-hypoxia brain damage. However, no studies have investigated NGB levels after ischaemic pre-conditioning and middle cerebral artery occlusion (MCAO).

Methods and procedures: This study subjected rats to different ischaemic pre-conditioning and MCAO regimens and assayed NGB levels in the hippocampus, cortex and hypothalamus by immunohistochemistry, quantitative polymerase chain reaction (PCR) and western blot.

Main outcomes and results: After 30 minutes of ischaemic pre-conditioning, the number of NGB-positive cells and NGB levels in the hippocampus, cortex and hypothalamus were increased with longer reperfusion times, peaked at 24-hours reperfusion and slightly decreased at 48-hours reperfusion. Similarly, the mRNA and protein expression levels of NGB were also up-regulated; they peaked at 24-hours reperfusion and slightly decreased at 48-hours reperfusion.

Conclusions: NGB may regulate neuroprotection against ischaemia and hypoxia-mediated brain damage after ischaemic pre-conditioning. The results provide additional evidence supporting the utility of ischaemic pre-conditioning and help elucidate its potential regulatory mechanism.

Keywords: Ischaemic pre-conditioning; middle cerebral artery occlusion; neuroglobin; neuroprotection; up-regulation.