Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2015 Jan 12:4:184.
doi: 10.3389/fcimb.2014.00184. eCollection 2014.

What RNAi screens in model organisms revealed about microbicidal response in mammals?

Prasad Abnave  1 , Filippo Conti  1 , Cedric Torre  1 , Eric Ghigo  1
Affiliations
Review

What RNAi screens in model organisms revealed about microbicidal response in mammals?

Prasad Abnave et al. Front Cell Infect Microbiol. .

Abstract

The strategies evolved by pathogens to infect hosts and the mechanisms used by the host to eliminate intruders are highly complex. Because several biological pathways and processes are conserved across model organisms, these organisms have been used for many years to elucidate and understand the mechanisms of the host-pathogen relationship and particularly to unravel the molecular processes enacted by the host to kill pathogens. The emergence of RNA interference (RNAi) and the ability to apply it toward studies in model organisms have allowed a breakthrough in the elucidation of host-pathogen interactions. The aim of this mini-review is to highlight and describe recent breakthroughs in the field of host-pathogen interactions using RNAi screens of model organisms. We will focus specifically on the model organisms Drosophila melanogaster, Caenorhabditis elegans, and Danio rerio. Moreover, a recent study examining the immune system of planarian will be discussed.

Keywords: C. elegans; D. melanogaster; D. rerio; RNA interference; host-pathogen interaction; innate immunity; orthologs.

PubMed Disclaimer

References

    1. Aballay A., Drenkard E., Hilbun L. R., Ausubel F. M. (2003). Caenorhabditis elegans innate immune response triggered by Salmonella enterica requires intact LPS and is mediated by a MAPK signaling pathway. Curr. Biol. 13, 47–52. 10.1016/S0960-9822(02)01396-9 - DOI - PubMed
    1. Abnave P., Mottola G., Gimenez G., Boucherit N., Trouplin V., Torre C., et al. . (2014). Screening in planarians identifies MORN2 as a key component in LC3-associated phagocytosis and resistance to bacterial infection. Cell Host Microbe 16, 338–350. 10.1016/j.chom.2014.08.002 - DOI - PubMed
    1. Agaisse H., Burrack L. S., Philips J. A., Rubin E. J., Perrimon N., Higgins D. E. (2005). Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Science 309, 1248–1251. 10.1126/science.1116008 - DOI - PubMed
    1. Baeg G.-H., Zhou R., Perrimon N. (2005). Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Genes Dev. 19, 1861–1870. 10.1101/gad.1320705 - DOI - PMC - PubMed
    1. Boutros M., Kiger A. A., Armknecht S., Kerr K., Hild M., Koch B., et al. . (2004). Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303, 832–835. 10.1126/science.1091266 - DOI - PubMed

LinkOut - more resources